Researchers from the University of Chicago have discovered a new way to collect samples to determine a patient’s pancreatic cancer, other than relying on peripheral blood from a more easily accessed vein in the arm.
From samples collected from the portal vein – which carries blood from the gastrointestinal tract, including from the pancreas, to the liver – physicians can learn far more about a patient’s pancreatic cancer.
Primary tumors shed cancerous cells, known as circulating tumor cells (CTCs), into the blood. These have been widely studied as prognostic biomarkers for various cancers.
The researchers posited that most cells released from a gastrointestinal tumor would flow into the portal vein and then get sequestered by the narrow vessels in the liver. These cells would not reach the peripheral venous system.
CTCs from gastrointestinal tumors are rarely identified in the peripheral blood until the cancer is widely metastatic.
The University of Chicago researchers used an ultrasound-guided endoscope and a small needle to take blood from the portal vein during routine diagnostic endoscopies.
They found CTCs in 100% of 18 patients with suspected tumors in the pancreas and bile ducts. Tests using peripheral blood samples, the standard method, detected tumors cells in only four of the 18 patients.
“We demonstrated that this method is potentially quite valuable as well as non-invasive, feasible and safe,” said study director Dr. Irving Waxman, professor of medicine and surgery and director of the Centre for Endoscopic Research and Therapeutics at the university. “We had no complications related to portal vein blood acquisition.”
The findings could offer doctors a method to diagnose pancreatic cancer earlier in patients. Only seven percent of patients diagnosed with stage II disease are still alive five years after diagnosis, making it one of the most lethal forms of cancer.
The portal vein samples contained far more tumor cells in all stages evaluated, including locally advanced as well as metastatic tumors, the researchers reported.
Blood collected from the portal vein had a mean of more than 100 CTCs per 7.5 milliliters. Patients with less advanced disease, who could potentially benefit from surgery to remove the tumor, had fewer CTCs. Those patients averaged about 80 CTCs per 7.5 milliliters.
In contrast, when researchers used peripheral blood to test the same patients, they found few, if any, circulating tumor cells. Those samples contained, on average, less than one CTC in 7.5 milliliters of blood, the equivalent of one cell in a billion.
“Access to circulating tumor cells may help us define the diagnosis and guide treatment,” Waxman said.
“In the setting of localized cancer where these findings are most applicable, the additional information of portal vein CTC number and their molecular characterization may help predict who will benefit from aggressive therapy before surgery, who is most at risk for a recurrence after the operation, and even who will not benefit from surgery at all,” said co-first author Dr. Daniel Catenacci, assistant professor of medicine at the University of Chicago.
These hidden cells in the portal venous system could help cancer specialists make better clinical decisions. Molecular characterization of CTCs at the time of diagnosis or after neoadjuvant therapy can provide clues about each patient’s prognosis.
“This is a novel and far more sensitive way to acquire, enumerate, and characterize CTCs from pancreatobiliary and other gastrointestinal cancers in this setting,” Waxman said. “We believe it can improve how we stratify patients.”
Patients who don’t have CTCs in the portal vein, for example, should have a better prognosis than those who do. Treatments can be personalized accordingly.
The authors agree that more studies need to be done to confirm their hypothesis with larger numbers of patients in a controlled setting. They plan further collaborative efforts.
“Ultimately, we envision that this new test could help plan treatment, based on a much more accurate record of the number and characteristics of circulating tumor cells,” said co-first author Dr. Christopher Chapman, a member of the Centre for Endoscopic Research and Therapeutics at the University of Chicago. “That should allow us to make better, more informed judgements about prognosis, and avoid interventions, such as surgery, that might not help.”